Veterinary Sciences and Medicine | Veterinary Journals

Abstract

Differential Detection of Brucella Canis by Means a Conventional Polymerase Chain Reaction

Author(s): Victoria C Lorca, Consuelo F Borie, Carlos O Navarro

The canine Brucellosis (BC) is an infectious, contagious and zoonotic disease caused by Brucella canis (B. canis). Nevertheless, there have been described sporadic infections caused also by Brucella sues and Brucella aborts as also for Brucella melitensis which produce an auto limited illness. This infection is characterized for producing infertility in males and females, affecting the reproductive life of the animal, which leads to important economic losses in breeding-kennels and affective loss for owners when the dog it carried to euthanasia. The diagnosis is usually realized by means serological tests whose principal disadvantage is the production of false positive results due to crossed reactivity with other bacteria, of the same or different genus, or false negative results in chronic infection cases and for this reason the diagnostic confirmation by means bacterial isolation is necessary. Nevertheless, the previous carries out risk of infection to the laboratory personnel due to zoonotic character of these bacteria. Additionally, this procedure involves a long period of incubation.

In consideration to the previous antecedents, the objective of this work was to develop a conventional PCR capable of differentiating between the detection of Brucella canis, Brucella sues and Brucella aborts, using the in silicon design of optimal primers, for generating a different and minor size fragment for Brucella sues with sequencing validation. Furthermore, the nucleotide sequences analysis was realized by means of free access programs.

This way, this method will constitute a promising alternative to the bacterial isolation as diagnostic direct and complementary method to serological tests.